Methods and Technologies

Molecular Epidemiology at the Centre for Arctic Health, has expertise and experience in a variety of methods and technologies for population studies, measurement of hormone disrupting effects in cell cultures in vitro and ex vivo (blood, urine, etc.) as well as measurements of oxidative stress and DNA changes.

  • Monitoring of accumulated chemicals (POPs such as PCBs, DDT / DDE, Perfluorinated Alkyl Substances [PFASs]) in the Greenlandic population.
  • Epidemiological studies focusing on POP exposure, lifestyle and disease vs. reproduction, embryonic development, cancer, obesity and allergies.
  • Population Studies which monitors and analyzes the effects of accumulated environmental chemicals such as PCBs and PFASs in pregnant women and their children.
  • Determination of the biological effects of serum POPs by SPE-HPLC fractionation.
  • Measurement of cellular oxidative stress.
  • Measuring DNA modifications (methylation, DNA breakage / damage).
  • Comparative arctic exposure studies among Inuit, animals and atmosphere.
  • Gene polymorphisms, gene expression and comparison of genotypes between different ethnic groups.
  • Comparison of genotypes between Inuit and Europeans.

The lab facilities at the Centre for Arctic Health & Molecular Epidemiology include mammalian cell culture laboratories with the needed equipment such as CO2 incubators, sterile bench, microscopes etc.. Instrumentation for general molecular biology and cellular oxidative stress analyses are available, facilities for SPE-HPLC extractions of human serum, Taq Man (Applied Biosystems) and Light Cycler for PCR or RT-Q-PCR and genotyping, Elisa Reader, luminometer and fluorometer for ER, AR and AhR gene-reporter analyses as well as fluorocytometer can be mentioned.